Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.9383 | 1.18409 | 3.03398 | 0.991828 | |
| 10day-seedling | 0.30599 | 0.177505 | 2.11415 | 0.0455422 | |
| 14day-seedling | 0.61653 | 0 | 2.61669 | 0 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | 0.49758 | 0.502044 | 2.32856 | 0.457501 | |
| 42day-rosette | 0.15039 | 0.558596 | 2.05778 | 0.885858 | 42day-stem | 0.22389 | 0.808458 | 1.94639 | 0.705465 |
| 53day-rosette | 0.40478 | 0.608257 | 2.24729 | 0.482081 | 53day-stem | 0.62635 | 0 | 2.02704 | 0 | 53day-inflores | 0.19883 | 0.810239 | 1.66064 | 0.8934 | 53day-silique | 2.84085 | 0.177577 | 3.98344 | 0.164945 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 38.69 | 10.71 | 5.96e-4 | -3.22 | 10.85 | 5.44e-4 | -3.26 | |
| 3 | 1.000 | 40.55 | 16.27 | 1.26e-5 | -4.9 | 15.5 | 2.15e-5 | -4.67 | |
| 10day-seedling | 1 | 1.000 | 34.52 | 21.66 | 3.02e-7 | -6.52 | 16.16 | 1.36e-5 | -4.87 |
| 2 | 1.000 | 35.46 | 22.22 | 2.05e-7 | -6.69 | 16.4 | 1.15e-5 | -4.94 | |
| 3 | 1.000 | 34.21 | 22.84 | 1.34e-7 | -6.87 | 16.12 | 1.40e-5 | -4.85 | |
| 14day-seedling | 1 | 0.999 | 34.78 | 21.21 | 4.14e-7 | -6.38 | 14.56 | 4.14e-5 | -4.38 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | 0.998 | 34.44 | 19.98 | 9.67e-7 | -6.01 | 14.44 | 4.49e-5 | -4.35 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 35.97 | 22.78 | 1.39e-7 | -6.86 | 16.59 | 1.01e-5 | -4.99 | |
| 42day-rosette | 1 | 0.995 | 34.98 | 21.44 | 3.51e-7 | -6.45 | 14.34 | 4.83e-5 | -4.32 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 37.59 | 24.07 | 5.69e-8 | -7.24 | 18.5 | 2.70e-6 | -5.57 | |
| 42day-stem | 1 | 0.992 | 34.27 | 22.58 | 1.59e-7 | -6.8 | 17.27 | 6.33e-6 | -5.2 |
| 2 | 0.999 | 32.33 | 19.79 | 1.13e-1 | -5.96 | 14.25 | 5.17e-1 | -4.29 | |
| 3 | 1.000 | 38.54 | 25.16 | 2.67e-8 | -7.57 | 18.85 | 2.11e-6 | -5.68 | |
| 53day-rosette | 1 | 1.000 | 34.46 | 20.41 | 7.18e-7 | -6.14 | 14.42 | 4.56e-5 | -4.34 |
| 2 | 1.000 | 33.4 | 21.11 | 4.41e-7 | -6.36 | 15.4 | 2.32e-5 | -4.63 | |
| 3 | 1.000 | 37.1 | 24.21 | 5.17e-8 | -7.29 | 17.55 | 5.21e-6 | -5.28 | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 35.95 | 21.17 | 4.23e-7 | -6.37 | 16.52 | 1.06e-5 | -4.97 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-inflores | 1 | 0.998 | 32.89 | 19.5 | 1.35e-6 | -5.87 | 14.34 | 4.81e-5 | -4.32 |
| 2 | 1.000 | 39.03 | 24.4 | 4.52e-8 | -7.34 | 19.84 | 1.06e-6 | -5.97 | |
| 3 | 0.980 | 37.44 | 23.62 | 7.78e-8 | -7.11 | 19.03 | 1.87e-6 | -5.73 | |
| 53day-silique | 1 | 1.000 | 33.88 | 14.03 | 5.96e-5 | -4.22 | 10.08 | 9.22e-4 | -3.04 |
| 2 | 1.000 | 32.44 | 13.15 | 1.10e-4 | -3.96 | 9.44 | 1.44e-3 | -2.84 | |
| 3 | 1.000 | 33.24 | 14.27 | 5.07e-5 | -4.29 | 10.53 | 6.74e-4 | -3.17 | |
NGS expression data
Expression of miR870 transcript in NGS dataSequences
miRNA transcript
CUUGCUUUAGAGACGAACGACACGAUUAUUCGUAAAGAAACAUUUGGUCUUCAGAAGAAGAAGAUUAACGGAGACGUUGCUGGCUCAGGUCACCGGGAAAAGCUUUUAUUUUUUUCUGGUCGAUCGAAGAACAUCAAAUUAGAAUGUGAUGCAAAACUUAGACUCCUAACAUUUGCAACACAAUCUAAUUUGGUGUUUCUUCGAUCUGGGUUCGUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAAUUUGGUGUUUCUUCGAUC | miRNA | 186 | 206 | |
TTGCTTTAGAGACGAACGACACG | primer | 5p | 2 | 24 |
ACGAACCCAGATCGAAGAAACA | primer | 3p | 194 | 215 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR870 found in PubMed |