Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.72188 | 0.441321 | 5.79439 | 0.577085 | |
| 10day-seedling | 4.44964 | 0.388191 | 6.257798 | 0.243633 | |
| 14day-seedling | 4.74742 | 0.54269 | 5.83686 | 0.397033 | |
| 25day-aerial | 4.50424 | 0.223611 | 6.284415 | 0.222911 | |
| 35day-aerial | 4.43865 | 0.180123 | 6.255808 | 0.0562726 | |
| 42day-rosette | 3.94416 | 0.645624 | 6.435669 | 0.972107 | 42day-stem | 4.973 | 0.132678 | 6.472726 | 0.254838 |
| 53day-rosette | 4.50821 | 0.392187 | 6.350712 | 0.393908 | 53day-stem | 5.06845 | 0.427814 | 6.613715 | 0.654306 | 53day-inflores | 5.32169 | 0.479729 | 6.891471 | 0.950293 | 53day-silique | 5.32312 | 0.0775273 | 6.465711 | 0.0373008 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28.02 | 4.17 | 5.57e-2 | -1.25 | 4.08 | 5.92e-2 | -1.23 |
| 2 | 1.000 | 33.73 | 5.75 | 1.86e-2 | -1.73 | 5.88 | 1.69e-2 | -1.77 | |
| 3 | 1.000 | 27.09 | 2.82 | 1.41e-1 | -0.85 | 2.05 | 2.41e-1 | -0.62 | |
| 10day-seedling | 1 | 1.000 | 19.89 | 7.03 | 7.63e-3 | -2.12 | 1.54 | 3.44e-1 | -0.46 |
| 2 | 1.000 | 22.1 | 8.86 | 2.16e-3 | -2.67 | 3.04 | 1.21e-1 | -0.92 | |
| 3 | 1.000 | 20.9 | 9.53 | 1.36e-3 | -2.87 | 2.81 | 1.42e-1 | -0.85 | |
| 14day-seedling | 1 | 1.000 | 22.54 | 8.97 | 1.99e-3 | -2.7 | 2.33 | 1.99e-1 | -0.7 |
| 2 | 0.996 | 25.95 | 7.83 | 4.38e-3 | -2.36 | 4.93 | 3.28e-2 | -1.48 | |
| 3 | 1.000 | 23.32 | 5.53 | 2.16e-2 | -1.66 | 3.39 | 9.53e-2 | -1.02 | |
| 25day-aerial | 1 | 1.000 | 21.83 | 8.97 | 1.99e-3 | -2.7 | 3.21 | 1.08e-1 | -0.97 |
| 2 | 1.000 | 22.09 | 8.04 | 3.79e-3 | -2.42 | 1.8 | 2.87e-1 | -0.54 | |
| 3 | 0.999 | 21.56 | 7.68 | 4.86e-3 | -2.31 | 2.12 | 2.30e-1 | -0.64 | |
| 35day-aerial | 1 | 0.999 | 22.28 | 7.82 | 4.43e-3 | -2.35 | 2.28 | 2.05e-1 | -0.69 |
| 2 | 0.999 | 21.51 | 8.79 | 2.26e-3 | -2.65 | 2.66 | 1.59e-1 | -0.8 | |
| 3 | 0.993 | 21.86 | 8.66 | 2.47e-3 | -2.61 | 2.48 | 1.80e-1 | -0.75 | |
| 42day-rosette | 1 | 0.996 | 20.47 | 6.93 | 8.18e-3 | -2.09 | -0.17 | 1.13e+0 | 0.05 |
| 2 | 1.000 | 24.6 | 10.22 | 8.37e-4 | -3.08 | 4.53 | 4.33e-2 | -1.36 | |
| 3 | 0.999 | 21.9 | 8.38 | 3.00e-3 | -2.52 | 2.81 | 1.42e-1 | -0.85 | |
| 42day-stem | 1 | 0.998 | 18.06 | 6.37 | 1.26e-1 | -1.92 | 1.63 | 4.79e-1 | -0.33 |
| 2 | 1.000 | 19.54 | 7.95 | 7.87e-1 | -2.17 | 1.45 | 3.66e-1 | -0.44 | |
| 3 | 1.000 | 19.96 | 6.58 | 1.47e-1 | -1.99 | 0.28 | 8.29e-1 | -0.81 | |
| 53day-rosette | 1 | 1.000 | 20.83 | 6.78 | 9.12e-3 | -2.04 | 0.79 | 5.79e-1 | -0.24 |
| 2 | 1.000 | 21.4 | 9.11 | 1.81e-3 | -2.74 | 3.39 | 9.51e-2 | -1.02 | |
| 3 | 1.000 | 21.84 | 8.95 | 2.03e-3 | -2.69 | 2.29 | 2.05e-1 | -0.69 | |
| 53day-stem | 1 | 0.981 | 18.78 | 5.35 | 2.44e-2 | -1.61 | -1.16 | 2.24e+0 | 0.35 |
| 2 | 0.997 | 21.55 | 6.78 | 9.12e-3 | -2.04 | 2.12 | 2.29e-1 | -0.64 | |
| 3 | 0.996 | 23.47 | 5.86 | 1.72e-2 | -1.77 | 2.91 | 1.33e-1 | -0.88 | |
| 53day-inflores | 1 | 0.997 | 20.8 | 7.41 | 5.87e-3 | -2.23 | 2.26 | 2.09e-1 | -0.68 |
| 2 | 0.999 | 19.39 | 4.76 | 3.70e-2 | -1.43 | 0.2 | 8.70e-1 | -0.06 | |
| 3 | 1.000 | 18.39 | 4.56 | 4.25e-2 | -1.37 | -0.03 | 1.02e+0 | 0.01 | |
| 53day-silique | 1 | 1.000 | 25.71 | 5.86 | 1.72e-2 | -1.76 | 1.91 | 2.66e-1 | -0.58 |
| 2 | 1.000 | 24.67 | 5.37 | 2.41e-2 | -1.62 | 1.67 | 3.14e-1 | -0.5 | |
| 3 | 1.000 | 24.45 | 5.47 | 2.25e-2 | -1.65 | 1.74 | 2.99e-1 | -0.52 | |
NGS expression data
Expression of miR775 transcript in NGS dataSequences
miRNA transcript
UUGGCGACUGAAUAAGAGGAUUUAAACGUUGCACUACGUGACAUUGAAACUGUCUUUCAACAUUCCAAUAUUUCAACUUUCGAAUACCCAAUAUUUGGUUUGUUCAAAGACAUUUUCGAUGUCUAGCAGUGCCAAUGUUUAAA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UUCGAUGUCUAGCAGUGCCA | miRNA | 115 | 134 | |
TGGCGACTGAATAAGAGGATTTAAAC | primer | 5p | 2 | 27 |
GGCACTGCTAGACATCGAAAATGT | primer | 3p | 110 | 133 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR775 found in PubMed |