Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.07401 | 0.313573 | 6.146522 | 0.422004 | |
| 10day-seedling | 4.79384 | 0.640446 | 6.602001 | 0.558778 | |
| 14day-seedling | 4.81405 | 0.499373 | 5.98686 | 0.226744 | |
| 25day-aerial | 4.55246 | 0.270909 | 6.304028 | 0.263135 | |
| 35day-aerial | 4.72337 | 0.300523 | 6.509465 | 0.208713 | |
| 42day-rosette | 4.60219 | 0.250048 | 6.445986 | 0.418387 | 42day-stem | 4.51857 | 0.0483701 | 6.239514 | 0.19936 |
| 53day-rosette | 5.12912 | 0.421831 | 6.9716254 | 0.409634 | 53day-stem | 4.60041 | 0.117464 | 6.114444 | 0.287494 | 53day-inflores | 4.90193 | 0.132293 | 6.359159 | 0.0876346 | 53day-silique | 4.74643 | 0.263532 | 5.88903 | 0.257879 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 26.31 | 2.45 | 1.83e-1 | -0.74 | 2.36 | 1.94e-1 | -0.71 |
| 2 | 1.000 | 32.26 | 4.28 | 5.15e-2 | -1.29 | 4.41 | 4.70e-2 | -1.33 | |
| 3 | 1.000 | 26.77 | 2.5 | 1.77e-1 | -0.75 | 1.73 | 3.01e-1 | -0.52 | |
| 10day-seedling | 1 | 1.000 | 17.78 | 4.92 | 3.31e-2 | -1.48 | -0.57 | 1.49e+0 | 0.17 |
| 2 | 1.000 | 22.2 | 8.95 | 2.03e-3 | -2.69 | 3.13 | 1.14e-1 | -0.94 | |
| 3 | 1.000 | 19.49 | 8.12 | 3.60e-3 | -2.44 | 1.41 | 3.77e-1 | -0.42 | |
| 14day-seedling | 1 | 0.999 | 22.72 | 9.15 | 1.77e-3 | -2.75 | 2.5 | 1.77e-1 | -0.75 |
| 2 | 0.999 | 24.73 | 6.62 | 1.02e-2 | -1.99 | 3.72 | 7.61e-2 | -1.12 | |
| 3 | 0.999 | 23.81 | 6.02 | 1.54e-2 | -1.81 | 3.88 | 6.80e-2 | -1.17 | |
| 25day-aerial | 1 | 0.997 | 19.96 | 7.1 | 7.29e-3 | -2.14 | 1.34 | 3.95e-1 | -0.4 |
| 2 | 0.999 | 22.58 | 8.53 | 2.70e-3 | -2.57 | 2.29 | 2.05e-1 | -0.69 | |
| 3 | 0.999 | 22.64 | 8.76 | 2.31e-3 | -2.64 | 3.2 | 1.09e-1 | -0.96 | |
| 35day-aerial | 1 | 0.995 | 20.99 | 6.54 | 1.07e-2 | -1.97 | 1 | 4.99e-1 | -0.3 |
| 2 | 0.998 | 21.26 | 8.53 | 2.70e-3 | -2.57 | 2.4 | 1.89e-1 | -0.72 | |
| 3 | 1.000 | 20.81 | 7.61 | 5.11e-3 | -2.29 | 1.43 | 3.72e-1 | -0.43 | |
| 42day-rosette | 1 | 0.999 | 21.09 | 7.55 | 5.32e-3 | -2.27 | 0.45 | 7.32e-1 | -0.14 |
| 2 | 0.999 | 23.3 | 8.92 | 2.06e-3 | -2.69 | 3.23 | 1.07e-1 | -0.97 | |
| 3 | 1.000 | 20.93 | 7.41 | 5.88e-3 | -2.23 | 1.84 | 2.79e-1 | -0.55 | |
| 42day-stem | 1 | 0.998 | 20.11 | 8.42 | 2.91e-1 | -2.54 | 3.11 | 1.16e-1 | -0.94 |
| 2 | 0.998 | 20.72 | 8.17 | 3.46e-1 | -2.47 | 2.62 | 1.62e-1 | -0.8 | |
| 3 | 1.000 | 21.5 | 8.12 | 3.67e-1 | -2.44 | 1.88 | 2.86e-1 | -0.54 | |
| 53day-rosette | 1 | 1.000 | 18.65 | 4.6 | 4.13e-2 | -1.38 | -1.39 | 2.62e+0 | 0.42 |
| 2 | 1.000 | 19.28 | 7 | 7.84e-3 | -2.11 | 1.28 | 4.12e-1 | -0.39 | |
| 3 | 1.000 | 19.94 | 7.05 | 7.53e-3 | -2.12 | 0.4 | 7.60e-1 | -0.12 | |
| 53day-stem | 1 | 0.999 | 21.81 | 8.39 | 2.99e-3 | -2.52 | 1.87 | 2.74e-1 | -0.56 |
| 2 | 1.000 | 22.69 | 7.91 | 4.15e-3 | -2.38 | 3.26 | 1.04e-1 | -0.98 | |
| 3 | 1.000 | 24.26 | 6.65 | 9.95e-3 | -2 | 3.7 | 7.71e-2 | -1.11 | |
| 53day-inflores | 1 | 1.000 | 20.62 | 7.22 | 6.69e-3 | -2.17 | 2.07 | 2.38e-1 | -0.62 |
| 2 | 1.000 | 21.63 | 7 | 7.81e-3 | -2.11 | 2.44 | 1.84e-1 | -0.74 | |
| 3 | 0.999 | 20.29 | 6.46 | 1.13e-2 | -1.95 | 1.87 | 2.73e-1 | -0.56 | |
| 53day-silique | 1 | 1.000 | 27.45 | 7.6 | 5.15e-3 | -2.29 | 3.65 | 7.96e-2 | -1.1 |
| 2 | 1.000 | 25.85 | 6.56 | 1.06e-2 | -1.97 | 2.85 | 1.38e-1 | -0.86 | |
| 3 | 1.000 | 27.27 | 8.3 | 3.18e-3 | -2.5 | 4.57 | 4.22e-2 | -1.37 | |
NGS expression data
Expression of miR408 transcript in NGS dataSequences
miRNA transcript
AAGGUUAGAUUGGUAUUGCAAUGAAAGAAGACAAAGCGGUAAUGAGAGAGAGACAGGGAACAAGCAGAGCAUGGAUUGAGUUUACUAAAACAUUAAACGACUCUGUUUUGUCUCUACCCAUGCACUGCCUCUUCCCUGGCUCCCUCUUUUUUUCUCUAUAUUUCUCUCUCUCCUUUCAUUUCACAGCUUUCAAUGGAAUUUUAUUGCUACUGCUAACG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
AUGCACUGCCUCUUCCCUGGC | miRNA | 120 | 140 | |
AAAGAAGACAAAGCGGTAATGAGAGA | primer | 5p | 24 | 49 |
AAAGAGGGAGCCAGGGAAGAG | primer | 3p | 129 | 149 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR408 found in PubMed |