miR399b
DB statistics:
  • Tuesday, April 7th 2020:
  • 114 miRNA familiesNews divider
  • 190 pre-miRNA sequencesNews divider
  • 7 developmental stages News divider
  • 190 hairpin structures
Expression
Switch to: Actin

In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).

The expression values are rescaled. Read more

Expression Data

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Expression Data
Development stageactinPP2A
LogSDLogSD
seed2.200091.131252.3290.985983
10day-seedling1.544730.8826433.352890.727091
14day-seedling-0.62701.374520
25day-aerial0.344670.5188162.088720.528418
35day-aerial0.577750.04697142.398380.141521
42day-rosette0.044490.5924311.956740.921257
42day-stem0.048960.7432392.214320.39156
53day-rosette0.446920.9882492.289430.894935
53day-stem0.597270.3900521.826980.491835
53day-inflores1.349680.3098852.781820.277254
53day-silique2.534590.5684683.677180.568899
Raw Expression Data

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Raw Data
Development stagerepR2CtactinPP2A
ΔCt2^(-ΔCt)log10ΔCt2^(-ΔCt)log10
seed11.00037.1413.291.00e-4-413.21.06e-4-3.97
2----N/D--N/D
31.00042.8718.62.51e-6-5.617.834.28e-6-5.37
10day-seedling11.00027.714.843.42e-5-4.479.341.54e-3-2.81
21.00032.3119.061.83e-6-5.7413.241.03e-4-3.99
31.00031.8420.476.87e-7-6.1613.767.21e-5-4.14
14day-seedling11.00037.0323.468.66e-8-7.0616.828.66e-6-5.06
2----N/D--N/D
3----N/D--N/D
25day-aerial10.99736.9524.15.58e-8-7.2518.343.02e-6-5.52
20.99635.2621.214.11e-7-6.3914.973.11e-5-4.51
31.00034.88214.78e-7-6.3215.432.26e-5-4.65
35day-aerial10.99835.6921.234.05e-7-6.3915.71.88e-5-4.73
20.99834.2421.513.34e-7-6.4815.382.34e-5-4.63
30.00034.1520.964.90e-7-6.3114.773.57e-5-4.45
42day-rosette10.99035.2321.692.95e-7-6.5314.594.06e-5-4.39
2----N/D--N/D
31.00038.0124.484.27e-8-7.3718.922.02e-6-5.69
42day-stem10.99932.8521.164.28e-7-6.3715.851.70e-1-4.78
21.00032.712.168.52e-1-6.714.613.99e-1-4.4
31.00036.923.528.34e-8-7.7917.286.64e-6-5.18
53day-rosette11.00037.923.856.63e-8-7.1817.864.21e-6-5.38
21.00030.2717.983.86e-6-5.4112.272.03e-4-3.69
31.00036.3723.488.57e-8-7.0716.828.65e-6-5.06
53day-stem10.99935.421.982.42e-7-6.6215.462.22e-5-4.65
20.99836.822.032.34e-7-6.6317.375.89e-6-5.23
30.00037.3819.771.12e-6-5.9516.828.64e-6-5.06
53day-inflores10.99932.7319.331.51e-6-5.8214.185.39e-5-4.27
20.99934.0219.391.45e-6-5.8414.843.41e-5-4.47
30.99731.4117.585.10e-6-5.2912.991.23e-4-3.91
53day-silique11.00034.5114.673.85e-5-4.4110.725.95e-4-3.23
21.00032.3313.031.19e-4-3.929.331.55e-3-2.81
31.00035.7716.88.76e-6-5.0613.071.16e-4-3.93
NGS expression data
Expression of miR399b transcript in NGS data
Sequences
miRNA transcript
UCACUAGUUUUAGGGCGCCUCUCCAUUGGCAGGUCCUUUACUUCCAAAUAUACACAUACAUAUAUGAAUAUCGAAAAUUUCCGAUGAUCGAUUUAUAAAUGACCUGCCAAAGGAGAGUUGCCCUGAAACUGGUUCAGCUUAAUUAAACACAUGAAAGAGAGAAGAAAUCAAAGG
Short sequences
sequencetypearmstartend
UGCCAAAGGAGAGUUGCCCUG
miRNA105125
CTCTCCATTGGCAGGTCCTTTA
primer5p1940
CCTTTGATTTCTTCTCTCTTTCATGTG
primer3p148174

For experimental procedure see:Materials and Methods


Cross-references
miRBasemiRBase record
ASRPASRP record
PMRDPMRD record
TAIRTAIR record
PubMed EntrezThe most up-to-date article(s) about miR399b found in PubMed

SHORTCUTS

STRUCTURE

miRNA
miRNA*
primer 5p
primer 3p
sRNA

Length = 174 nt
MFE = -61.00 kcal/mol

COMMENTS