Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.20009 | 1.13125 | 2.329 | 0.985983 | |
| 10day-seedling | 1.54473 | 0.882643 | 3.35289 | 0.727091 | |
| 14day-seedling | -0.627 | 0 | 1.37452 | 0 | |
| 25day-aerial | 0.34467 | 0.518816 | 2.08872 | 0.528418 | |
| 35day-aerial | 0.57775 | 0.0469714 | 2.39838 | 0.141521 | |
| 42day-rosette | 0.04449 | 0.592431 | 1.95674 | 0.921257 | 42day-stem | 0.04896 | 0.743239 | 2.21432 | 0.39156 |
| 53day-rosette | 0.44692 | 0.988249 | 2.28943 | 0.894935 | 53day-stem | 0.59727 | 0.390052 | 1.82698 | 0.491835 | 53day-inflores | 1.34968 | 0.309885 | 2.78182 | 0.277254 | 53day-silique | 2.53459 | 0.568468 | 3.67718 | 0.568899 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 37.14 | 13.29 | 1.00e-4 | -4 | 13.2 | 1.06e-4 | -3.97 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 42.87 | 18.6 | 2.51e-6 | -5.6 | 17.83 | 4.28e-6 | -5.37 | |
| 10day-seedling | 1 | 1.000 | 27.7 | 14.84 | 3.42e-5 | -4.47 | 9.34 | 1.54e-3 | -2.81 |
| 2 | 1.000 | 32.31 | 19.06 | 1.83e-6 | -5.74 | 13.24 | 1.03e-4 | -3.99 | |
| 3 | 1.000 | 31.84 | 20.47 | 6.87e-7 | -6.16 | 13.76 | 7.21e-5 | -4.14 | |
| 14day-seedling | 1 | 1.000 | 37.03 | 23.46 | 8.66e-8 | -7.06 | 16.82 | 8.66e-6 | -5.06 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 0.997 | 36.95 | 24.1 | 5.58e-8 | -7.25 | 18.34 | 3.02e-6 | -5.52 |
| 2 | 0.996 | 35.26 | 21.21 | 4.11e-7 | -6.39 | 14.97 | 3.11e-5 | -4.51 | |
| 3 | 1.000 | 34.88 | 21 | 4.78e-7 | -6.32 | 15.43 | 2.26e-5 | -4.65 | |
| 35day-aerial | 1 | 0.998 | 35.69 | 21.23 | 4.05e-7 | -6.39 | 15.7 | 1.88e-5 | -4.73 |
| 2 | 0.998 | 34.24 | 21.51 | 3.34e-7 | -6.48 | 15.38 | 2.34e-5 | -4.63 | |
| 3 | 0.000 | 34.15 | 20.96 | 4.90e-7 | -6.31 | 14.77 | 3.57e-5 | -4.45 | |
| 42day-rosette | 1 | 0.990 | 35.23 | 21.69 | 2.95e-7 | -6.53 | 14.59 | 4.06e-5 | -4.39 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 38.01 | 24.48 | 4.27e-8 | -7.37 | 18.92 | 2.02e-6 | -5.69 | |
| 42day-stem | 1 | 0.999 | 32.85 | 21.16 | 4.28e-7 | -6.37 | 15.85 | 1.70e-1 | -4.78 |
| 2 | 1.000 | 32.71 | 2.16 | 8.52e-1 | -6.7 | 14.61 | 3.99e-1 | -4.4 | |
| 3 | 1.000 | 36.9 | 23.52 | 8.34e-8 | -7.79 | 17.28 | 6.64e-6 | -5.18 | |
| 53day-rosette | 1 | 1.000 | 37.9 | 23.85 | 6.63e-8 | -7.18 | 17.86 | 4.21e-6 | -5.38 |
| 2 | 1.000 | 30.27 | 17.98 | 3.86e-6 | -5.41 | 12.27 | 2.03e-4 | -3.69 | |
| 3 | 1.000 | 36.37 | 23.48 | 8.57e-8 | -7.07 | 16.82 | 8.65e-6 | -5.06 | |
| 53day-stem | 1 | 0.999 | 35.4 | 21.98 | 2.42e-7 | -6.62 | 15.46 | 2.22e-5 | -4.65 |
| 2 | 0.998 | 36.8 | 22.03 | 2.34e-7 | -6.63 | 17.37 | 5.89e-6 | -5.23 | |
| 3 | 0.000 | 37.38 | 19.77 | 1.12e-6 | -5.95 | 16.82 | 8.64e-6 | -5.06 | |
| 53day-inflores | 1 | 0.999 | 32.73 | 19.33 | 1.51e-6 | -5.82 | 14.18 | 5.39e-5 | -4.27 |
| 2 | 0.999 | 34.02 | 19.39 | 1.45e-6 | -5.84 | 14.84 | 3.41e-5 | -4.47 | |
| 3 | 0.997 | 31.41 | 17.58 | 5.10e-6 | -5.29 | 12.99 | 1.23e-4 | -3.91 | |
| 53day-silique | 1 | 1.000 | 34.51 | 14.67 | 3.85e-5 | -4.41 | 10.72 | 5.95e-4 | -3.23 |
| 2 | 1.000 | 32.33 | 13.03 | 1.19e-4 | -3.92 | 9.33 | 1.55e-3 | -2.81 | |
| 3 | 1.000 | 35.77 | 16.8 | 8.76e-6 | -5.06 | 13.07 | 1.16e-4 | -3.93 | |
NGS expression data
Expression of miR399b transcript in NGS dataSequences
miRNA transcript
UCACUAGUUUUAGGGCGCCUCUCCAUUGGCAGGUCCUUUACUUCCAAAUAUACACAUACAUAUAUGAAUAUCGAAAAUUUCCGAUGAUCGAUUUAUAAAUGACCUGCCAAAGGAGAGUUGCCCUGAAACUGGUUCAGCUUAAUUAAACACAUGAAAGAGAGAAGAAAUCAAAGG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAGUUGCCCUG | miRNA | 105 | 125 | |
CTCTCCATTGGCAGGTCCTTTA | primer | 5p | 19 | 40 |
CCTTTGATTTCTTCTCTCTTTCATGTG | primer | 3p | 148 | 174 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399b found in PubMed |