Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.89942 | 1.29373 | 3.97193 | 1.42882 | |
| 10day-seedling | 4.3953 | 0.225394 | 6.203452 | 0.21344 | |
| 14day-seedling | 4.85722 | 0.560665 | 6.030369 | 0.432567 | |
| 25day-aerial | 4.66762 | 0.216493 | 6.430595 | 0.114897 | |
| 35day-aerial | 4.91148 | 0.302873 | 6.699784 | 0.136232 | |
| 42day-rosette | 5.14717 | 0.306184 | 7.0290677 | 0.435778 | 42day-stem | 4.81216 | 0.581186 | 6.775258 | 0.0387718 |
| 53day-rosette | 5.49076 | 0.204229 | 7.333261 | 0.131261 | 53day-stem | 5.26598 | 0.26921 | 6.452857 | 0.240712 | 53day-inflores | 5.29948 | 0.151436 | 6.744274 | 0.103992 | 53day-silique | 4.94752 | 0.114817 | 6.090118 | 0.0768881 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.97 | 10.12 | 9.01e-4 | -3.05 | 10.03 | 9.57e-4 | -3.02 |
| 2 | 1.000 | 42.66 | 14.69 | 3.79e-5 | -4.42 | 14.82 | 3.46e-5 | -4.46 | |
| 3 | 1.000 | 30.37 | 6.1 | 1.46e-2 | -1.84 | 5.33 | 2.49e-2 | -1.6 | |
| 10day-seedling | 1 | 1.000 | 20.68 | 7.82 | 4.42e-3 | -2.35 | 2.33 | 1.99e-1 | -0.7 |
| 2 | 1.000 | 22.52 | 9.27 | 1.62e-3 | -2.79 | 3.46 | 9.10e-2 | -1.04 | |
| 3 | 1.000 | 20.23 | 8.87 | 2.14e-3 | -2.67 | 2.15 | 2.25e-1 | -0.65 | |
| 14day-seedling | 1 | 0.996 | 22.18 | 8.61 | 2.57e-3 | -2.59 | 1.96 | 2.57e-1 | -0.59 |
| 2 | 0.997 | 25.81 | 7.7 | 4.82e-3 | -2.32 | 4.79 | 3.61e-2 | -1.44 | |
| 3 | 1.000 | 22.82 | 5.03 | 3.05e-2 | -1.52 | 2.9 | 1.34e-1 | -0.87 | |
| 25day-aerial | 1 | 0.999 | 20.57 | 7.71 | 4.78e-3 | -2.32 | 1.95 | 2.59e-1 | -0.59 |
| 2 | 0.994 | 22.53 | 8.48 | 2.79e-3 | -2.55 | 2.24 | 2.11e-1 | -0.67 | |
| 3 | 1.000 | 20.93 | 7.05 | 7.56e-3 | -2.12 | 1.48 | 3.57e-1 | -0.45 | |
| 35day-aerial | 1 | 0.995 | 20.33 | 5.87 | 1.71e-2 | -1.77 | 0.33 | 7.93e-1 | -0.1 |
| 2 | 1.000 | 19.79 | 7.07 | 7.43e-3 | -2.13 | 0.94 | 5.22e-1 | -0.28 | |
| 3 | 0.993 | 20.86 | 7.66 | 4.93e-3 | -2.31 | 1.48 | 3.60e-1 | -0.44 | |
| 42day-rosette | 1 | 1.000 | 19.77 | 6.23 | 1.33e-2 | -1.88 | -0.87 | 1.83e+0 | 0.26 |
| 2 | 1.000 | 19.38 | 4.99 | 3.14e-2 | -1.5 | -0.7 | 1.62e+0 | 0.21 | |
| 3 | 1.000 | 20.65 | 7.13 | 7.14e-3 | -2.15 | 1.56 | 3.39e-1 | -0.47 | |
| 42day-stem | 1 | 1.000 | 17.74 | 6.49 | 1.52e-1 | -1.83 | 0.74 | 5.99e-1 | -0.22 |
| 2 | 0.999 | 18.78 | 6.23 | 1.33e-1 | -1.88 | 0.69 | 6.22e-1 | -0.26 | |
| 3 | 1.000 | 20.31 | 6.93 | 8.27e-1 | -2.86 | 0.62 | 6.50e-1 | -0.19 | |
| 53day-rosette | 1 | 1.000 | 18.43 | 4.38 | 4.79e-2 | -1.32 | -1.6 | 3.04e+0 | 0.48 |
| 2 | 1.000 | 17.21 | 4.92 | 3.29e-2 | -1.48 | -0.79 | 1.73e+0 | 0.24 | |
| 3 | 1.000 | 18.62 | 5.73 | 1.88e-2 | -1.73 | -0.92 | 1.90e+0 | 0.28 | |
| 53day-stem | 1 | 0.997 | 19.96 | 6.53 | 1.08e-2 | -1.97 | - | - | N/D |
| 2 | 0.999 | 19.56 | 4.78 | 3.64e-2 | -1.44 | 0.13 | 9.15e-1 | -0.04 | |
| 3 | 0.999 | 23.28 | 5.68 | 1.95e-2 | -1.71 | 2.73 | 1.51e-1 | -0.82 | |
| 53day-inflores | 1 | 0.999 | 19.38 | 5.99 | 1.57e-2 | -1.8 | 0.84 | 5.60e-1 | -0.25 |
| 2 | 0.999 | 20.39 | 5.76 | 1.85e-2 | -1.73 | 1.2 | 4.35e-1 | -0.36 | |
| 3 | 0.994 | 18.93 | 5.1 | 2.91e-2 | -1.54 | 0.51 | 7.02e-1 | -0.15 | |
| 53day-silique | 1 | 1.000 | 27.07 | 7.22 | 6.69e-3 | -2.17 | 3.27 | 1.03e-1 | -0.99 |
| 2 | 1.000 | 25.76 | 6.47 | 1.13e-2 | -1.95 | 2.76 | 1.47e-1 | -0.83 | |
| 3 | 1.000 | 25.73 | 6.76 | 9.21e-3 | -2.04 | 3.03 | 1.22e-1 | -0.91 | |
NGS expression data
Expression of miR162a transcript in NGS dataSequences
miRNA transcript
UAGUUGGAAGAAGAGUGAGAGUCGCUGGAGGCAGCGGUUCAUCGAUCUCUUCCUGUGAACACAUUAAAAAUGUAAAAGCAUGAAUAGAUCGAUAAACCUCUGCAUCCAGCGUUUGCCUCUUGUAUCUUUCUUAUUGACUUGAAUCUUUUUGUUUAUUGGUUUUUGAAAGAAGAAAAAAGUGAGGGAAAACAGAGUCAAGUCCGAUUACUUUUCUGGGAAAGCUGAGGAUUUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGAUAAACCUCUGCAUCCAG | miRNA | 89 | 109 | |
AGAAGAGTGAGAGTCGCTGGA | primer | 5p | 9 | 29 |
AAATCCTCAGCTTTCCCAGA | primer | 3p | 212 | 231 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR162a found in PubMed |