Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 7.46793 | 0.359239 | 7.54044 | 0.488698 | |
| 10day-seedling | 5.51954 | 0.420135 | 7.3277 | 0.291271 | |
| 14day-seedling | 5.4906 | 0.457423 | 6.66395 | 0.580587 | |
| 25day-aerial | 5.61885 | 0.198033 | 7.41948 | 0.0900417 | |
| 35day-aerial | 5.4733 | 0.22495 | 7.37096 | 0.285342 | |
| 42day-rosette | 5.49948 | 0.415291 | 7.29913 | 0.538939 | 42day-stem | 5.54446 | 0.183288 | 7.26213 | 0.252108 |
| 53day-rosette | 5.71603 | 0.663706 | 7.55854 | 0.642759 | 53day-stem | 5.74852 | 0.262739 | 6.94606 | 0.586916 | 53day-inflores | 5.96968 | 0.226546 | 7.4071 | 0.135249 | 53day-silique | 6.1683 | 0.0980731 | 7.31089 | 0.0665165 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28.66 | 4.81 | 3.57e-2 | -1.45 | 4.72 | 3.80e-2 | -1.42 |
| 2 | 1.000 | 34.38 | 6.4 | 1.19e-2 | -1.93 | 6.53 | 1.08e-2 | -1.97 | |
| 3 | 1.000 | 28.34 | 4.06 | 5.98e-2 | -1.22 | 3.29 | 1.02e-1 | -0.99 | |
| 10day-seedling | 1 | 1.000 | 22.83 | 9.97 | 9.98e-4 | -3 | 4.47 | 4.50e-2 | -1.35 |
| 2 | 1.000 | 25.4 | 12.15 | 2.20e-4 | -3.66 | 6.34 | 1.23e-2 | -1.91 | |
| 3 | 1.000 | 23.93 | 12.57 | 1.65e-4 | -3.78 | 5.85 | 1.73e-2 | -1.76 | |
| 14day-seedling | 1 | 0.999 | 26.04 | 12.47 | 1.76e-4 | -3.75 | 5.83 | 1.76e-2 | -1.75 |
| 2 | 0.000 | 30.7 | 12.59 | 1.62e-4 | -3.79 | 9.69 | 1.21e-3 | -2.92 | |
| 3 | 0.997 | 27.69 | 9.9 | 1.04e-3 | -2.98 | 7.77 | 4.59e-3 | -2.34 | |
| 25day-aerial | 1 | 1.000 | 23.52 | 10.66 | 6.16e-4 | -3.21 | 4.91 | 3.34e-2 | -1.48 |
| 2 | 0.996 | 25.7 | 11.66 | 3.09e-4 | -3.51 | 5.42 | 2.34e-2 | -1.63 | |
| 3 | 0.998 | 24.85 | 10.97 | 4.98e-4 | -3.3 | 5.41 | 2.35e-2 | -1.63 | |
| 35day-aerial | 1 | 0.999 | 24.56 | 10.11 | 9.07e-4 | -3.04 | 4.57 | 4.21e-2 | -1.38 |
| 2 | 0.999 | 25.29 | 12.57 | 1.64e-4 | -3.78 | 6.44 | 1.15e-2 | -1.94 | |
| 3 | 0.214 | 24.39 | 11.19 | 4.27e-4 | -3.37 | 5.01 | 3.11e-2 | -1.51 | |
| 42day-rosette | 1 | 0.998 | 24.3 | 10.76 | 5.76e-4 | -3.24 | 3.66 | 7.93e-2 | -1.1 |
| 2 | 0.998 | 27.6 | 13.22 | 1.05e-4 | -3.98 | 7.53 | 5.42e-3 | -2.27 | |
| 3 | 1.000 | 24.42 | 10.9 | 5.25e-4 | -3.28 | 5.33 | 2.49e-2 | -1.6 | |
| 42day-stem | 1 | 0.996 | 22.73 | 11.4 | 4.75e-1 | -3.32 | 5.73 | 1.88e-1 | -1.72 |
| 2 | 0.997 | 24.7 | 12.16 | 2.19e-1 | -3.66 | 6.62 | 1.23e-1 | -2 | |
| 3 | 0.998 | 24.37 | 1.99 | 4.92e-1 | -3.38 | 4.68 | 3.90e-1 | -1.49 | |
| 53day-rosette | 1 | 1.000 | 22.41 | 8.36 | 3.04e-3 | -2.52 | 2.37 | 1.93e-1 | -0.71 |
| 2 | 1.000 | 24.43 | 12.15 | 2.21e-4 | -3.66 | 6.43 | 1.16e-2 | -1.94 | |
| 3 | 1.000 | 25.11 | 12.22 | 2.10e-4 | -3.68 | 5.56 | 2.12e-2 | -1.67 | |
| 53day-stem | 1 | 0.999 | 24.94 | 11.52 | 3.42e-4 | -3.47 | 5 | 3.13e-2 | -1.5 |
| 2 | 0.998 | 25.84 | 11.06 | 4.68e-4 | -3.33 | 6.41 | 1.18e-2 | -1.93 | |
| 3 | 0.999 | 27.43 | 9.83 | 1.10e-3 | -2.96 | 6.87 | 8.52e-3 | -2.07 | |
| 53day-inflores | 1 | 0.998 | 24.29 | 10.9 | 5.23e-4 | -3.28 | 5.75 | 1.86e-2 | -1.73 |
| 2 | 1.000 | 24.46 | 9.84 | 1.09e-3 | -2.96 | 5.28 | 2.57e-2 | -1.59 | |
| 3 | 0.992 | 23.27 | 9.44 | 1.44e-3 | -2.84 | 4.85 | 3.47e-2 | -1.46 | |
| 53day-silique | 1 | 1.000 | 29.56 | 9.71 | 1.20e-3 | -2.92 | 5.76 | 1.85e-2 | -1.73 |
| 2 | 1.000 | 28.35 | 9.06 | 1.87e-3 | -2.73 | 5.36 | 2.44e-2 | -1.61 | |
| 3 | 1.000 | 28.42 | 9.45 | 1.43e-3 | -2.84 | 5.72 | 1.90e-2 | -1.72 | |
NGS expression data
Expression of miR161 transcript in NGS dataSequences
miRNA transcript
UGCUUGAUCUCGGUUUUUGACCAGUUUAUUGCGUCGAUCAAUGCAUUGAAAGUGACUACAUCGGGGUUCCGAUUUUUUUUGUUCUUCAUAUGAUGAAGCGGAAACAGUAAUCAACCCUGGUUUAGUCACUUUCACUGCAUUAAUCAAUGCAUUUGUAAAAAGAGGGAAAAGCA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAAAGUGACUACAUCGGGGU | miRNA | 47 | 67 | |
UCAAUGCAUUGAAAGUGACUA | miRNA | 38 | 58 | |
TGACCAGTTTATTGCGTCGATCA | primer | 5p | 18 | 40 |
TGCTTTTCCCTCTTTTTACAAATGC | primer | 3p | 149 | 173 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR161 found in PubMed |