No amplification after 40 cycles; cDNA concentration was the same as for others analysed pri-miRNAs
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Limit for selected miRNAs number is 24. Following miRNA are not shown: 823, 822, 319a, 319c, 319b, 862, 393a, 393b, 391, 390a, 390b, 397a, 397b, 396a, 396b, 395a, 395c, 395b, 395e, 395d, 395f, 394a, 394b, 399a, 399c, 399b, 399e, 399d, 399f, 398a, 398c, 398b, 833, 830, 831, 837, 834, 835, 838, 839, 420, 447a, 447c, 447b, 847, 846, 845a, 844, 843, 842, 841, 783, 848, 173, 172a, 172c, 172b, 172e, 172d, 171a, 171c, 171b, 170, 854a, 854c, 854b, 854d, 855, 856, 857, 850, 851, 852, 853, 858, 2111a, 2111b, 2112, 781, 168a, 168b, 169a, 169c, 169b, 169e, 169d, 169f, 169i, 169h, 169k, 169j, 169m, 169l, 169n, 164a, 164c, 164b, 165a, 165b, 166a, 166c, 166b, 166e, 166d, 166g, 166f, 167a, 167c, 167b, 167d, 160a, 160c, 160b, 161, 162a, 162b, 163, 869, 868, 782, 861, 860, 863, 840, 865, 864, 866, 157a, 157c, 157b, 157d, 156a, 156c, 156b, 156e, 156d, 156g, 156f, 156h, 159a, 159b, 158a, 870, 775, 774, 777, 776, 771, 773, 779, 1888, 1887, 1886, 472. Show all (NOT RECOMMENDED).
| miRNA | 53day-stem |
|---|---|
| 400 | 4.45796 |
| 401 | 4.50239 |
| 402 | 5.16706 |
| 403 | 6.227606 |
| 404 | 1.92623 |
| 405a | 3.2553 |
| 405b | 3.30737 |
| 405d | 4.76656 |
| 406 | 3.58227 |
| 408 | 6.114444 |
| 413 | 4.29313 |
| 414 | 7.875254 |
| 415 | 6.01988 |
| 416 | 4.88544 |
| 417 | 3.98564 |
| 418 | 3.67755 |
| 419 | 5.91127 |
| 780 | 6.513954 |
| 824 | 5.71729 |
| 825 | 6.480193 |
| 826 | 5.99223 |
| 827 | 5.05556 |
| 828 | 5.28743 |
| 829 | 2.61919 |
Low quality data are indicated in gray. Read more
Text in grey indicates low quality data, i.e. correlation coefficient of expression values from all three replicas is below 0.995.