Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.68577 | 0.178542 | 6.81467 | 0.323813 | |
| 10day-seedling | 7.00745 | 0.314124 | 8.815603 | 0.175093 | |
| 14day-seedling | 7.90989 | 0.52108 | 9.127279 | 0.395721 | |
| 25day-aerial | 7.62238 | 0.190032 | 9.563427 | 0.19682 | |
| 35day-aerial | 7.58703 | 0.538942 | 9.378053 | 0.467938 | |
| 42day-rosette | 6.88081 | 0.882774 | 9.141494 | 0.786967 | 42day-stem | 6.78744 | 0.343622 | 8.8703 | 0.725074 |
| 53day-rosette | 7.21798 | 0.391432 | 9.0604885 | 0.332676 | 53day-stem | 6.90899 | 0.589556 | 8.35187 | 0.55998 | 53day-inflores | 6.11269 | 0.252872 | 7.5791 | 0.315998 | 53day-silique | 5.81942 | 0.194156 | 6.96202 | 0.223011 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.96 | 8.11 | 3.63e-3 | -2.44 | 8.02 | 3.85e-3 | -2.41 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 31.54 | 7.27 | 6.49e-3 | -2.19 | 6.5 | 1.11e-2 | -1.96 | |
| 10day-seedling | 1 | 1.000 | 18.32 | 5.46 | 2.27e-2 | -1.64 | -0.03 | 1.02e+0 | 0.01 |
| 2 | 1.000 | 20.16 | 6.91 | 8.32e-3 | -2.08 | 1.1 | 4.68e-1 | -0.33 | |
| 3 | 1.000 | 18.86 | 7.49 | 5.58e-3 | -2.25 | 0.77 | 5.85e-1 | -0.23 | |
| 14day-seedling | 1 | 0.999 | 18.58 | 5.01 | 3.11e-2 | -1.51 | -1.64 | 3.11e+0 | 0.49 |
| 2 | 0.000 | 21.98 | 3.87 | 6.84e-2 | -1.16 | 0.96 | 5.13e-1 | -0.29 | |
| 3 | 0.994 | 19.57 | 1.78 | 2.91e-1 | -0.54 | -0.36 | 1.28e+0 | 0.11 | |
| 25day-aerial | 1 | 0.998 | 16.24 | 3.38 | 9.62e-2 | -1.02 | -2.38 | 5.21e+0 | 0.72 |
| 2 | 0.997 | 19.15 | 5.11 | 2.90e-2 | -1.54 | -1.13 | 2.20e+0 | 0.34 | |
| 3 | 0.996 | 17.34 | 3.46 | 9.06e-2 | -1.04 | -2.1 | 4.28e+0 | 0.63 | |
| 35day-aerial | 1 | 1.000 | 17.35 | 2.89 | 1.35e-1 | -0.87 | -2.64 | 6.25e+0 | 0.8 |
| 2 | 0.999 | 19.2 | 6.47 | 1.12e-2 | -1.95 | 0.34 | 7.89e-1 | -0.1 | |
| 3 | 0.997 | 17.85 | 4.66 | 3.96e-2 | -1.4 | -1.53 | 2.89e+0 | 0.46 | |
| 42day-rosette | 1 | 1.000 | 20.44 | 6.9 | 8.39e-3 | -2.08 | -0.21 | 1.16e+0 | 0.06 |
| 2 | 0.995 | 22.62 | 8.24 | 3.32e-3 | -2.48 | 2.54 | 1.71e-1 | -0.77 | |
| 3 | 1.000 | 17.22 | 3.7 | 7.70e-2 | -1.11 | -1.87 | 3.65e+0 | 0.56 | |
| 42day-stem | 1 | 0.999 | 18.47 | 6.78 | 9.12e-1 | -2.49 | 1.47 | 3.62e-1 | -0.44 |
| 2 | 0.994 | 20.24 | 7.7 | 4.82e-1 | -2.32 | 2.15 | 2.26e-1 | -0.65 | |
| 3 | 1.000 | 19.46 | 6.75 | 1.48e-1 | -1.83 | -0.23 | 1.17e+0 | 0.7 | |
| 53day-rosette | 1 | 1.000 | 18.56 | 4.51 | 4.38e-2 | -1.36 | -1.48 | 2.78e+0 | 0.44 |
| 2 | 1.000 | 18.46 | 6.17 | 1.39e-2 | -1.86 | 0.45 | 7.30e-1 | -0.14 | |
| 3 | 1.000 | 19.97 | 7.08 | 7.41e-3 | -2.13 | 0.42 | 7.48e-1 | -0.13 | |
| 53day-stem | 1 | 1.000 | 20.49 | 7.06 | 7.48e-3 | -2.13 | 0.54 | 6.86e-1 | -0.16 |
| 2 | 0.993 | 23.62 | 8.84 | 2.18e-3 | -2.66 | 4.19 | 5.48e-2 | -1.26 | |
| 3 | 1.000 | 22.28 | 4.68 | 3.90e-2 | -1.41 | 1.72 | 3.03e-1 | -0.52 | |
| 53day-inflores | 1 | 0.993 | 22.71 | 9.32 | 1.57e-3 | -2.8 | 4.16 | 5.58e-2 | -1.25 |
| 2 | 1.000 | 25.12 | 10.49 | 6.97e-4 | -3.16 | 5.93 | 1.64e-2 | -1.79 | |
| 3 | 1.000 | 22.48 | 8.66 | 2.48e-3 | -2.61 | 4.07 | 5.97e-2 | -1.22 | |
| 53day-silique | 1 | 1.000 | 29.87 | 10.02 | 9.63e-4 | -3.02 | 6.07 | 1.49e-2 | -1.83 |
| 2 | 1.000 | 29.69 | 10.4 | 7.40e-4 | -3.13 | 6.69 | 9.65e-3 | -2.02 | |
| 3 | 1.000 | 30.25 | 11.28 | 4.03e-4 | -3.39 | 7.55 | 5.35e-3 | -2.27 | |
NGS expression data
Expression of miR850 transcript in NGS dataSequences
miRNA transcript
UUUUAGAUAGUGUUAUGUAUUUAUUUCUUACCUAUAGAAACCUGUCAGCUAAGAUCCGGACUACAACAAAGCACGUCUACUCUACUAUAUUCUUUUGCGAUAAUCAAUAGAUUCUUCUAACAUCGUAAAGGAAGACAAAAGAGUAUACAUACUUAGUUGUCGUCGUAUCUUUGUUGAGAAGUUUCUAAAUGGCACAUAGAAAGUACAUAAAACUAUAUGAA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAAGAUCCGGACUACAACAAAG | miRNA | 50 | 71 | |
CGGACTACAACAAAGCACGTCTACT | primer | 5p | 57 | 81 |
TTCTCAACAAAGATACGACGACAACT | primer | 3p | 155 | 180 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR850 found in PubMed |