Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.7323 | 0.0732286 | 5.80481 | 0.165228 | |
| 10day-seedling | 3.95184 | 0.0629274 | 5.76 | 0.138664 | |
| 14day-seedling | 3.86305 | 0.319876 | 5.0783 | 0.405535 | |
| 25day-aerial | 3.3065 | 0.378144 | 5.35531 | 0.316715 | |
| 35day-aerial | 3.48259 | 0.288077 | 5.5275 | 0.070241 | |
| 42day-rosette | 3.45857 | 0.169468 | 5.30129 | 0.414975 | 42day-stem | 3.60828 | 0.133403 | 5.35629 | 0.0325753 |
| 53day-rosette | 3.74569 | 0.328754 | 5.58819 | 0.212419 | 53day-stem | 4.12537 | 0.661459 | 5.54064 | 0.218755 | 53day-inflores | 4.06981 | 0.0514576 | 5.50472 | 0.0683476 | 53day-silique | 4.47597 | 0.0922515 | 5.61856 | 0.132299 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 27.81 | 3.95 | 6.47e-2 | -1.19 | 3.86 | 6.87e-2 | -1.16 |
| 2 | 1.000 | 32.41 | 4.43 | 4.64e-2 | -1.33 | 4.56 | 4.22e-2 | -1.37 | |
| 3 | 1.000 | 28.52 | 4.25 | 5.25e-2 | -1.28 | 3.48 | 8.94e-2 | -1.05 | |
| 10day-seedling | 1 | 1.000 | 22.75 | 9.89 | 1.05e-3 | -2.98 | 4.4 | 4.74e-2 | -1.32 |
| 2 | 1.000 | 23.43 | 10.18 | 8.60e-4 | -3.07 | 4.37 | 4.84e-2 | -1.32 | |
| 3 | 1.000 | 21.67 | 10.3 | 7.93e-4 | -3.1 | 3.59 | 8.32e-2 | -1.08 | |
| 14day-seedling | 1 | 1.000 | 25.03 | 11.46 | 3.54e-4 | -3.45 | 4.82 | 3.55e-2 | -1.45 |
| 2 | 0.988 | 28.12 | 10.01 | 9.68e-4 | -3.01 | 7.11 | 7.25e-3 | -2.14 | |
| 3 | 0.990 | 27.14 | 9.35 | 1.53e-3 | -2.82 | 7.21 | 6.73e-3 | -2.17 | |
| 25day-aerial | 1 | 0.997 | 23.14 | 10.28 | 8.03e-4 | -3.1 | 4.52 | 4.35e-2 | -1.36 |
| 2 | 0.999 | 26.88 | 12.84 | 1.36e-4 | -3.87 | 6.6 | 1.03e-2 | -1.99 | |
| 3 | 0.999 | 24.71 | 10.83 | 5.50e-4 | -3.26 | 5.27 | 2.60e-2 | -1.59 | |
| 35day-aerial | 1 | 1.000 | 24.7 | 10.25 | 8.24e-4 | -3.08 | 4.71 | 3.82e-2 | -1.42 |
| 2 | 0.999 | 23.67 | 10.94 | 5.07e-4 | -3.29 | 4.81 | 3.56e-2 | -1.45 | |
| 3 | 0.998 | 24.53 | 11.34 | 3.85e-4 | -3.41 | 5.15 | 2.81e-2 | -1.55 | |
| 42day-rosette | 1 | 0.999 | 24.71 | 11.17 | 4.35e-4 | -3.36 | 4.06 | 5.99e-2 | -1.22 |
| 2 | 0.999 | 26.67 | 12.29 | 2.00e-4 | -3.7 | 6.59 | 1.04e-2 | -1.98 | |
| 3 | 1.000 | 25.36 | 11.84 | 2.73e-4 | -3.56 | 6.27 | 1.29e-2 | -1.89 | |
| 42day-stem | 1 | 0.999 | 22.35 | 1.66 | 6.19e-1 | -3.29 | 5.35 | 2.45e-1 | -1.61 |
| 2 | 1.000 | 23.66 | 11.12 | 4.50e-1 | -3.35 | 5.57 | 2.16e-1 | -1.68 | |
| 3 | 1.000 | 25.15 | 11.77 | 2.87e-1 | -3.54 | 5.46 | 2.27e-1 | -1.64 | |
| 53day-rosette | 1 | 1.000 | 24.01 | 9.96 | 1.00e-3 | -3 | 3.98 | 6.36e-2 | -1.2 |
| 2 | 1.000 | 22.71 | 10.42 | 7.28e-4 | -3.14 | 4.71 | 3.83e-2 | -1.42 | |
| 3 | 1.000 | 24.93 | 12.04 | 2.37e-4 | -3.63 | 5.39 | 2.39e-2 | -1.62 | |
| 53day-stem | 1 | 0.999 | 25.46 | 12.04 | 2.38e-4 | -3.62 | 5.52 | 2.18e-2 | -1.66 |
| 2 | 1.000 | 23.52 | 8.74 | 2.33e-3 | -2.63 | 4.09 | 5.87e-2 | -1.23 | |
| 3 | 1.000 | 25.47 | 7.87 | 4.28e-3 | -2.37 | 4.91 | 3.31e-2 | -1.48 | |
| 53day-inflores | 1 | 0.999 | 23.29 | 9.9 | 1.05e-3 | -2.98 | 4.74 | 3.74e-2 | -1.43 |
| 2 | 0.999 | 24.38 | 9.75 | 1.16e-3 | -2.94 | 5.2 | 2.73e-2 | -1.56 | |
| 3 | 1.000 | 23.38 | 9.55 | 1.33e-3 | -2.88 | 4.96 | 3.20e-2 | -1.49 | |
| 53day-silique | 1 | 1.000 | 27.89 | 8.05 | 3.78e-3 | -2.42 | 4.1 | 5.85e-2 | -1.23 |
| 2 | 1.000 | 27.94 | 8.64 | 2.50e-3 | -2.6 | 4.94 | 3.26e-2 | -1.49 | |
| 3 | 1.000 | 27.44 | 8.46 | 2.83e-3 | -2.55 | 4.73 | 3.76e-2 | -1.42 | |
NGS expression data
Expression of miR830 transcript in NGS dataSequences
miRNA transcript
CAGAGUCUCGCUAGUGUCUUUACUAAACACAAAAUCUGUAAACGCCUCGAUCUCCUCUUCUCCAAAUAGUUUAGGUUAGCUGACAUAUAAUAAUUCAAUAGACCCCAGUUUCUCCAGAUAACCUAACUAUUUUGAGAAGAAGUGAACGAAGAGCGAUUACAAGUUAGGAGAGUGCUUGAAUUUUGAAGAACAUCG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAACUAUUUUGAGAAGAAGUG | miRNA | 124 | 144 | |
TGTAAACGCCTCGATCTCCTCTTCT | primer | 5p | 37 | 61 |
ATCGCTCTTCGTTCACTTCTTCTC | primer | 3p | 134 | 157 |
UCUUCUCCAAAUAGUUUAGGUU | miRNA* | * | 56 | 77 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR830 found in PubMed |