Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 7.05847 | 0.361145 | 7.13099 | 0.4877 | |
| 10day-seedling | 5.39363 | 0.471444 | 7.20179 | 0.281225 | |
| 14day-seedling | 5.75446 | 0.372937 | 6.92714 | 0.385409 | |
| 25day-aerial | 4.97082 | 0.629959 | 6.88819 | 0.16215 | |
| 35day-aerial | 5.31506 | 0.176031 | 6.99353 | 0.329067 | |
| 42day-rosette | 5.4312 | 0.181153 | 6.95773 | 0.819844 | 42day-stem | 5.54119 | 0.0599188 | 7.25922 | 0.187086 |
| 53day-rosette | 5.53449 | 0.276269 | 7.377 | 0.169513 | 53day-stem | 5.65529 | 0.620144 | 7.28743 | 0.0362138 | 53day-inflores | 5.66383 | 0.423708 | 7.3133 | 0.100347 | 53day-silique | 5.95402 | 0.202025 | 7.09662 | 0.240911 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 29.95 | 6.09 | 1.47e-2 | -1.83 | 6 | 1.56e-2 | -1.81 |
| 2 | 1.000 | 35.77 | 7.79 | 4.53e-3 | -2.34 | 7.92 | 4.12e-3 | -2.38 | |
| 3 | 1.000 | 29.74 | 5.47 | 2.26e-2 | -1.65 | 4.7 | 3.85e-2 | -1.42 | |
| 10day-seedling | 1 | 1.000 | 23.59 | 10.73 | 5.90e-4 | -3.23 | 5.23 | 2.66e-2 | -1.58 |
| 2 | 1.000 | 24.73 | 11.48 | 3.51e-4 | -3.45 | 5.66 | 1.97e-2 | -1.71 | |
| 3 | 1.000 | 25.1 | 13.74 | 7.33e-5 | -4.13 | 7.02 | 7.69e-3 | -2.11 | |
| 14day-seedling | 1 | 0.998 | 25.64 | 12.06 | 2.33e-4 | -3.63 | 5.42 | 2.34e-2 | -1.63 |
| 2 | 0.999 | 28.8 | 10.69 | 6.06e-4 | -3.22 | 7.78 | 4.54e-3 | -2.34 | |
| 3 | 1.000 | 27.38 | 9.59 | 1.30e-3 | -2.89 | 7.45 | 5.71e-3 | -2.24 | |
| 25day-aerial | 1 | 1.000 | 24.94 | 12.08 | 2.31e-4 | -3.64 | 6.32 | 1.25e-2 | -1.9 |
| 2 | 0.990 | 27.58 | 13.54 | 8.40e-5 | -4.08 | 7.3 | 6.36e-3 | -2.2 | |
| 3 | 0.999 | 26.16 | 12.28 | 2.02e-4 | -3.7 | 6.71 | 9.53e-3 | -2.02 | |
| 35day-aerial | 1 | 1.000 | 26.26 | 11.8 | 2.80e-4 | -3.55 | 6.27 | 1.30e-2 | -1.89 |
| 2 | 1.000 | 25.62 | 12.9 | 1.31e-4 | -3.88 | 6.77 | 9.16e-3 | -2.04 | |
| 3 | 0.998 | 25.21 | 12.01 | 2.42e-4 | -3.62 | 5.83 | 1.76e-2 | -1.75 | |
| 42day-rosette | 1 | 1.000 | 25.2 | 11.67 | 3.08e-4 | -3.51 | 4.56 | 4.24e-2 | -1.37 |
| 2 | 0.999 | 25.75 | 11.37 | 3.77e-4 | -3.42 | 5.68 | 1.95e-2 | -1.71 | |
| 3 | 1.000 | 26.05 | 12.53 | 1.69e-4 | -3.77 | 6.96 | 8.02e-3 | -2.1 | |
| 42day-stem | 1 | 0.999 | 22.99 | 11.33 | 3.96e-1 | -3.42 | 5.99 | 1.57e-1 | -1.84 |
| 2 | 0.997 | 24.26 | 11.72 | 2.96e-1 | -3.53 | 6.18 | 1.39e-1 | -1.86 | |
| 3 | 1.000 | 24.76 | 11.37 | 3.78e-1 | -3.42 | 5.66 | 2.99e-1 | -1.53 | |
| 53day-rosette | 1 | 1.000 | 24.81 | 10.76 | 5.75e-4 | -3.24 | 4.78 | 3.65e-2 | -1.44 |
| 2 | 1.000 | 23.52 | 11.24 | 4.15e-4 | -3.38 | 5.52 | 2.18e-2 | -1.66 | |
| 3 | 1.000 | 25.43 | 12.54 | 1.68e-4 | -3.77 | 5.88 | 1.70e-2 | -1.77 | |
| 53day-stem | 1 | 1.000 | 25.66 | 12.23 | 2.07e-4 | -3.68 | 5.72 | 1.90e-2 | -1.72 |
| 2 | 0.999 | 24.98 | 10.21 | 8.47e-4 | -3.07 | 5.55 | 2.13e-2 | -1.67 | |
| 3 | 1.000 | 26.34 | 8.73 | 2.35e-3 | -2.63 | 5.78 | 1.82e-2 | -1.74 | |
| 53day-inflores | 1 | 1.000 | 24.18 | 10.79 | 5.66e-4 | -3.25 | 5.63 | 2.02e-2 | -1.7 |
| 2 | 1.000 | 24.86 | 10.23 | 8.32e-4 | -3.08 | 5.67 | 1.96e-2 | -1.71 | |
| 3 | 0.986 | 23.68 | 9.85 | 1.09e-3 | -2.96 | 5.26 | 2.62e-2 | -1.58 | |
| 53day-silique | 1 | 1.000 | 29.21 | 9.36 | 1.52e-3 | -2.82 | 5.41 | 2.35e-2 | -1.63 |
| 2 | 1.000 | 29.64 | 10.35 | 7.65e-4 | -3.12 | 6.65 | 9.97e-3 | -2 | |
| 3 | 1.000 | 29.61 | 10.64 | 6.26e-4 | -3.2 | 6.91 | 8.32e-3 | -2.08 | |
NGS expression data
Expression of miR828 transcript in NGS dataSequences
miRNA transcript
GUGACAAAGUCACAAAAGUGAUAAACUCUCCUUUCCACUUUCUAAUGUUUCUUGCUUAAAUGAGUAUUCCAUGUAAAAAUGAUUCACUCACUCGUAUGGGUGGGUUGGGUUUUCUCUUCAUGAGAUGCUCAUUUGAGCAAGCAAUGUUUGGAAGUGGGACUAUUUAAUAUCAUGUAUGAGUUUUAUCUUUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCUUGCUUAAAUGAGUAUUCCA | miRNA | 50 | 71 | |
CCTTTCCACTTTCTAATGTTTCTTGCT | primer | 5p | 30 | 56 |
ATAGTCCCACTTCCAAACATTGCTT | primer | 3p | 139 | 163 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR828 found in PubMed |