Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.38915 | 0.69337 | 5.46166 | 0.835033 | |
| 10day-seedling | 2.74307 | 1.56322 | 4.58036 | 1.30381 | |
| 14day-seedling | 4.06418 | 0 | 6.06562 | 0 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | 3.96554 | 0.405552 | 5.71539 | 0.523005 | |
| 42day-rosette | 4.10352 | 0.816438 | 6.1731 | 1.31932 | 42day-stem | 5.46352 | 0.177305 | 7.18149 | 0.0858615 |
| 53day-rosette | 2.57716 | 0.76106 | 4.41967 | 0.906638 | 53day-stem | 4.04845 | 0.880656 | 4.82598 | 1.51249 | 53day-inflores | 6.83063 | 0.330203 | 8.2581 | 0.281549 | 53day-silique | 6.56467 | 0.40048 | 7.70727 | 0.432552 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 36.7 | 12.84 | 1.36e-4 | -3.87 | 12.76 | 1.45e-4 | -3.84 |
| 2 | 1.000 | 41.73 | 13.75 | 7.23e-5 | -4.14 | 13.89 | 6.59e-5 | -4.18 | |
| 3 | 1.000 | 33.66 | 9.39 | 1.49e-3 | -2.83 | 8.62 | 2.54e-3 | -2.59 | |
| 10day-seedling | 1 | 1.000 | 29.97 | 17.11 | 7.05e-6 | -5.15 | 11.62 | 3.18e-4 | -3.5 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 35.83 | 24.46 | 4.34e-8 | -7.36 | 17.74 | 4.55e-6 | -5.34 | |
| 14day-seedling | 1 | 1.000 | 29.95 | 16.38 | 1.17e-5 | -4.93 | 9.73 | 1.17e-3 | -2.93 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | 1.000 | 32.13 | 17.68 | 4.77e-6 | -5.32 | 12.14 | 2.22e-4 | -3.65 |
| 2 | 0.984 | 28.49 | 15.77 | 1.79e-5 | -4.75 | 9.64 | 1.25e-3 | -2.9 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | 0.991 | 26.93 | 13.39 | 9.29e-5 | -4.03 | 6.29 | 1.28e-2 | -1.89 |
| 2 | 0.999 | 32.56 | 18.18 | 3.36e-6 | -5.47 | 12.49 | 1.74e-4 | -3.76 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-stem | 1 | 0.997 | 23.01 | 11.33 | 4.00e-1 | -3.48 | 6.16 | 1.55e-1 | -1.89 |
| 2 | 1.000 | 23.82 | 11.28 | 4.24e-1 | -3.4 | 5.73 | 1.88e-1 | -1.72 | |
| 3 | 0.999 | 25.79 | 12.49 | 1.84e-1 | -3.74 | 6.12 | 1.46e-1 | -1.84 | |
| 53day-rosette | 1 | 1.000 | 35.93 | 21.88 | 2.59e-7 | -6.59 | 15.89 | 1.65e-5 | -4.78 |
| 2 | 1.000 | 35.84 | 23.55 | 8.15e-8 | -7.09 | 17.83 | 4.28e-6 | -5.37 | |
| 3 | 1.000 | 31.47 | 18.58 | 2.55e-6 | -5.59 | 11.92 | 2.57e-4 | -3.59 | |
| 53day-stem | 1 | 1.000 | 26.84 | 13.42 | 9.14e-5 | -4.04 | 6.9 | 8.38e-3 | -2.08 |
| 2 | 1.000 | 29.72 | 14.94 | 3.17e-5 | -4.5 | 10.29 | 7.98e-4 | -3.1 | |
| 3 | 0.000 | 37.43 | 19.82 | 1.08e-6 | -5.97 | 16.87 | 8.37e-6 | -5.08 | |
| 53day-inflores | 1 | 0.994 | 21.48 | 8.09 | 3.68e-3 | -2.43 | 2.93 | 1.31e-1 | -0.88 |
| 2 | 0.999 | 22.18 | 7.55 | 5.32e-3 | -2.27 | 3 | 1.25e-1 | -0.9 | |
| 3 | 0.991 | 19.81 | 5.98 | 1.59e-2 | -1.8 | 1.39 | 3.82e-1 | -0.42 | |
| 53day-silique | 1 | 1.000 | 26.86 | 7.01 | 7.75e-3 | -2.11 | 3.06 | 1.20e-1 | -0.92 |
| 2 | 1.000 | 28.87 | 9.58 | 1.31e-3 | -2.88 | 5.87 | 1.71e-2 | -1.77 | |
| 3 | 1.000 | 26.65 | 7.68 | 4.87e-3 | -2.31 | 3.95 | 6.47e-2 | -1.19 | |
NGS expression data
Expression of miR776 transcript in NGS dataSequences
miRNA transcript
ACAAAUCCAUCGGAACCCUAAACAUCUCCUUGUGGCAGAGUUAUGACUUUUUACUUCCAGUGAUGAAAUUUGGUUGGGAUUAUAGCCACGAACUCAAUAGAAUUCUUAAGAUCAUGUGAAAACGUUGACAAGAUCGUCUACGAUUUUCCAAGAUUCUAAGUCUUCUAUUGAUGUUCAUGGCUUUAACCUGAUUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCUAAGUCUUCUAUUGAUGUU | miRNA | 155 | 175 | |
CAAATCCATCGGAACCCTAAACA | primer | 5p | 2 | 24 |
AATCAGGTTAAAGCCATGAACATCAA | primer | 3p | 168 | 193 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR776 found in PubMed |