Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.96545 | 0.256624 | 6.037959 | 0.123991 | |
| 10day-seedling | 5.59286 | 0.482164 | 7.401018 | 0.340333 | |
| 14day-seedling | 5.88351 | 0.673218 | 7.185284 | 0.35822 | |
| 25day-aerial | 5.86193 | 0.380813 | 7.639506 | 0.31405 | |
| 35day-aerial | 5.58336 | 0.685582 | 7.794758 | 0.606406 | |
| 42day-rosette | 5.83904 | 0.286928 | 7.768056 | 0.0653109 | 42day-stem | 4.86926 | 0.650539 | 6.85428 | 0.358883 |
| 53day-rosette | 6.408355 | 0.387081 | 8.25086 | 0.365999 | 53day-stem | 5.62518 | 0.462852 | 7.142738 | 0.237087 | 53day-inflores | 4.91356 | 0.169901 | 6.39654 | 0.0708635 | 53day-silique | 5.71939 | 0.0969597 | 6.861989 | 0.0694974 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 26.79 | 2.94 | 1.31e-1 | -0.88 | 2.85 | 1.39e-1 | -0.86 |
| 2 | 1.000 | 30.93 | 2.95 | 1.29e-1 | -0.89 | 3.09 | 1.18e-1 | -0.93 | |
| 3 | 1.000 | 28.69 | 4.42 | 4.67e-2 | -1.33 | 3.65 | 7.96e-2 | -1.1 | |
| 10day-seedling | 1 | 1.000 | 15.72 | 2.86 | 1.37e-1 | -0.86 | -2.63 | 6.19e+0 | 0.79 |
| 2 | 1.000 | 18.5 | 5.25 | 2.62e-2 | -1.58 | -0.56 | 1.48e+0 | 0.17 | |
| 3 | 1.000 | 17.28 | 5.91 | 1.67e-2 | -1.78 | -0.81 | 1.75e+0 | 0.24 | |
| 14day-seedling | 1 | 0.998 | 19.8 | 6.23 | 1.34e-2 | -1.87 | -0.42 | 1.34e+0 | 0.13 |
| 2 | 0.994 | 21.06 | 2.95 | 1.29e-1 | -0.89 | 0.05 | 9.68e-1 | -0.01 | |
| 3 | 0.999 | 19.74 | 1.95 | 2.59e-1 | -0.59 | -0.19 | 1.14e+0 | 0.06 | |
| 25day-aerial | 1 | 0.999 | 15.35 | 2.5 | 1.77e-1 | -0.75 | -3.26 | 9.61e+0 | 0.98 |
| 2 | 1.000 | 19.07 | 5.03 | 3.07e-2 | -1.51 | -1.22 | 2.32e+0 | 0.37 | |
| 3 | 1.000 | 17.55 | 3.67 | 7.85e-2 | -1.1 | -1.89 | 3.71e+0 | 0.57 | |
| 35day-aerial | 1 | 0.999 | 16.55 | 2.1 | 2.34e-1 | -0.63 | -3.44 | 1.09e+1 | 1.04 |
| 2 | 0.998 | 18.32 | 5.6 | 2.07e-2 | -1.68 | -0.54 | 1.45e+0 | 0.16 | |
| 3 | 1.000 | 16.55 | 3.35 | 9.79e-2 | -1.01 | -2.83 | 7.13e+0 | 0.85 | |
| 42day-rosette | 1 | 1.000 | 18.29 | 4.75 | 3.72e-2 | -1.43 | -2.36 | 5.12e+0 | 0.71 |
| 2 | 1.000 | 17.77 | 3.39 | 9.56e-2 | -1.02 | -2.31 | 4.94e+0 | 0.69 | |
| 3 | 0.999 | 16.38 | 2.85 | 1.38e-1 | -0.86 | -2.72 | 6.57e+0 | 0.82 | |
| 42day-stem | 1 | 0.998 | 18.63 | 6.93 | 8.17e-1 | -2.88 | 1.62 | 3.24e-1 | -0.49 |
| 2 | 0.999 | 18.67 | 6.13 | 1.43e-1 | -1.85 | 0.59 | 6.69e-1 | -0.17 | |
| 3 | 0.999 | 18.94 | 5.55 | 2.13e-1 | -1.67 | -0.75 | 1.69e+0 | 0.23 | |
| 53day-rosette | 1 | 1.000 | 14.54 | 0.49 | 7.13e-1 | -0.15 | -5.5 | 4.53e+1 | 1.66 |
| 2 | 1.000 | 14.87 | 2.58 | 1.67e-1 | -0.78 | -3.14 | 8.80e+0 | 0.94 | |
| 3 | 1.000 | 15.72 | 2.83 | 1.41e-1 | -0.85 | -3.83 | 1.42e+1 | 1.15 | |
| 53day-stem | 1 | 1.000 | 19.22 | 5.8 | 1.80e-2 | -1.75 | -0.72 | 1.65e+0 | 0.22 |
| 2 | 0.999 | 19.84 | 5.06 | 3.00e-2 | -1.52 | 0.41 | 7.54e-1 | -0.12 | |
| 3 | 0.999 | 20.45 | 2.84 | 1.39e-1 | -0.86 | -0.11 | 1.08e+0 | 0.03 | |
| 53day-inflores | 1 | 0.999 | 20.71 | 7.31 | 6.28e-3 | -2.2 | 2.16 | 2.24e-1 | -0.65 |
| 2 | 1.000 | 20.92 | 6.29 | 1.28e-2 | -1.89 | 1.74 | 3.00e-1 | -0.52 | |
| 3 | 1.000 | 20.52 | 6.69 | 9.67e-3 | -2.01 | 2.1 | 2.33e-1 | -0.63 | |
| 53day-silique | 1 | 1.000 | 24.37 | 4.52 | 4.35e-2 | -1.36 | 0.57 | 6.72e-1 | -0.17 |
| 2 | 1.000 | 23.19 | 3.9 | 6.71e-2 | -1.17 | 0.19 | 8.75e-1 | -0.06 | |
| 3 | 1.000 | 23.31 | 4.34 | 4.93e-2 | -1.31 | 0.61 | 6.55e-1 | -0.18 | |
NGS expression data
Expression of miR398b transcript in NGS dataSequences
miRNA transcript
AGUGGAUCUCGACAGGGUUGAUAUGAGAACACACGAGUAAUCAACGGCUGUAAUGACGCUACGUCAUUGUUACAGCUCUCGUUUUCAUGUGUUCUCAGGUCACCCCUGCUGAGCUCUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGUGUUCUCAGGUCACCCCUG | miRNA | 88 | 108 | |
AGTGGATCTCGACAGGGTTG | primer | 5p | 1 | 20 |
GGGGTGACCTGAGAACACAT | primer | 3p | 87 | 106 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR398b found in PubMed |