Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.38444 | 1.55871 | 3.45695 | 1.44955 | |
| 10day-seedling | 1.25061 | 0.601422 | 3.08791 | 0.34201 | |
| 14day-seedling | 2.43225 | 0.220974 | 3.60479 | 0.806061 | |
| 25day-aerial | 1.16798 | 0.551552 | 2.9305 | 0.460536 | |
| 35day-aerial | 1.36148 | 0.480585 | 3.21583 | 0.46909 | |
| 42day-rosette | 1.78163 | 0.745568 | 3.59028 | 0.364514 | 42day-stem | 1.6299 | 0.0676655 | 3.26447 | 0.0169775 |
| 53day-rosette | 1.67926 | 0.0637053 | 3.52176 | 0.181692 | 53day-stem | 2.36441 | 0.960485 | 3.78184 | 0.544819 | 53day-inflores | 1.5952 | 0.611768 | 3.18464 | 0.64463 | 53day-silique | 2.92703 | 1.09849 | 4.06962 | 1.13554 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.01 | 7.15 | 7.04e-3 | -2.15 | 7.06 | 7.48e-3 | -2.13 |
| 2 | 1.000 | 39.4 | 11.43 | 3.63e-4 | -3.44 | 11.56 | 3.31e-4 | -3.48 | |
| 3 | 1.000 | 41.73 | 17.46 | 5.56e-6 | -5.25 | 16.69 | 9.48e-6 | -5.02 | |
| 10day-seedling | 1 | 1.000 | 30.55 | 17.69 | 4.74e-6 | -5.32 | 12.19 | 2.14e-4 | -3.67 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 31.88 | 20.51 | 6.69e-7 | -6.17 | 13.8 | 7.02e-5 | -4.15 | |
| 14day-seedling | 1 | 1.000 | 28.68 | 15.11 | 2.83e-5 | -4.55 | 8.46 | 2.83e-3 | -2.55 |
| 2 | 0.995 | 32.58 | 14.47 | 4.39e-5 | -4.36 | 11.57 | 3.29e-4 | -3.48 | |
| 3 | 0.998 | 33.73 | 15.94 | 1.59e-5 | -4.8 | 13.8 | 7.02e-5 | -4.15 | |
| 25day-aerial | 1 | 0.999 | 30.84 | 17.98 | 3.87e-6 | -5.41 | 12.22 | 2.09e-4 | -3.68 |
| 2 | 0.998 | 35.49 | 21.45 | 3.49e-7 | -6.46 | 15.21 | 2.65e-5 | -4.58 | |
| 3 | 1.000 | 32.57 | 18.69 | 2.36e-6 | -5.63 | 13.13 | 1.12e-4 | -3.95 | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 30.32 | 17.6 | 5.03e-6 | -5.3 | 11.47 | 3.53e-4 | -3.45 | |
| 3 | 1.000 | 33.05 | 19.86 | 1.05e-6 | -5.98 | 13.67 | 7.66e-5 | -4.12 | |
| 42day-rosette | 1 | 0.998 | 32.63 | 19.09 | 1.80e-6 | -5.75 | 11.98 | 2.47e-4 | -3.61 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 29.11 | 15.58 | 2.04e-5 | -4.69 | 10.02 | 9.66e-4 | -3.02 | |
| 42day-stem | 1 | 1.000 | 29.37 | 17.68 | 4.76e-1 | -5.32 | 12.37 | 1.90e-1 | -3.72 |
| 2 | 0.997 | 30.54 | 18 | 3.82e-1 | -5.42 | 12.45 | 1.79e-1 | -3.75 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | 1.000 | 31.96 | 17.92 | 4.05e-6 | -5.39 | 11.93 | 2.57e-4 | -3.59 |
| 2 | 1.000 | 29.88 | 17.6 | 5.05e-6 | -5.3 | 11.88 | 2.65e-4 | -3.58 | |
| 3 | 1.000 | 30.41 | 17.52 | 5.34e-6 | -5.27 | 10.86 | 5.39e-4 | -3.27 | |
| 53day-stem | 1 | 0.999 | 30.88 | 17.46 | 5.56e-6 | -5.25 | 10.94 | 5.10e-4 | -3.29 |
| 2 | 1.000 | 31.79 | 17.02 | 7.54e-6 | -5.12 | 12.36 | 1.90e-4 | -3.72 | |
| 3 | 1.000 | 29.33 | 11.72 | 2.96e-4 | -3.53 | 8.77 | 2.29e-3 | -2.64 | |
| 53day-inflores | 1 | 0.999 | 30.18 | 16.79 | 8.82e-6 | -5.05 | 11.64 | 3.14e-4 | -3.5 |
| 2 | 1.000 | 30.36 | 15.73 | 1.84e-5 | -4.74 | 11.18 | 4.32e-4 | -3.36 | |
| 3 | 0.994 | 33.55 | 19.72 | 1.16e-6 | -5.94 | 15.13 | 2.79e-5 | -4.55 | |
| 53day-silique | 1 | 1.000 | 29.74 | 9.89 | 1.05e-3 | -2.98 | 5.94 | 1.62e-2 | -1.79 |
| 2 | 1.000 | 36.48 | 17.19 | 6.68e-6 | -5.18 | 13.49 | 8.70e-5 | -4.06 | |
| 3 | 1.000 | 32.47 | 13.5 | 8.61e-5 | -4.06 | 9.77 | 1.14e-3 | -2.94 | |
NGS expression data
Expression of miR166d transcript in NGS dataSequences
miRNA transcript
UGGUCAUUUGCUCCUCUCUCCUAUGUAUAAAUAUUCGCCUCACACAUAGACCUAUUUAGCUUCUUCUUCUUCUUCUUCUUCUGUCUACUUACAUAAAGUUAUCCUGCUUUGGUUUAGGGUUGAGAGGAAUAUUGUCUGGCUCGAGGUCAUGAAGAAGAUCGGUAGAUUGAUUCAUUUUAAAGAGUGAAAUCCCUAAAUGAUUCUCGGACCAGGCUUCAUUCCCCCCAACC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 204 | 224 | |
GGTCATTTGCTCCTCTCTCCTATGT | primer | 5p | 2 | 26 |
TCCTCTCAACCCTAAACCAAAGC | primer | 3p | 106 | 128 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166d found in PubMed |