Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.8928 | 0.764258 | 6.96531 | 0.683309 | |
| 10day-seedling | 6.10381 | 0.436206 | 7.91197 | 0.441842 | |
| 14day-seedling | 6.21166 | 0.66052 | 7.48608 | 0.127499 | |
| 25day-aerial | 5.72047 | 0.0665708 | 7.46772 | 0.0489343 | |
| 35day-aerial | 5.54257 | 0.117292 | 7.50475 | 0.102323 | |
| 42day-rosette | 5.36633 | 0.551134 | 7.27841 | 0.55466 | 42day-stem | 5.66794 | 0.13849 | 7.39056 | 0.16229 |
| 53day-rosette | 5.16579 | 0.716959 | 7.0083 | 0.657711 | 53day-stem | 5.97915 | 1.19817 | 7.41375 | 0.889925 | 53day-inflores | 5.94979 | 0.166407 | 7.38336 | 0.235173 | 53day-silique | 5.79258 | 0.374892 | 6.93517 | 0.41505 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 32.51 | 8.65 | 2.49e-3 | -2.6 | 8.56 | 2.64e-3 | -2.58 |
| 2 | 1.000 | 32.05 | 4.08 | 5.93e-2 | -1.23 | 4.21 | 5.40e-2 | -1.27 | |
| 3 | 1.000 | 32.55 | 8.27 | 3.23e-3 | -2.49 | 7.5 | 5.51e-3 | -2.26 | |
| 10day-seedling | 1 | 1.000 | 21.09 | 8.23 | 3.33e-3 | -2.48 | 2.74 | 1.50e-1 | -0.82 |
| 2 | 1.000 | 24.37 | 11.12 | 4.49e-4 | -3.35 | 5.31 | 2.52e-2 | -1.6 | |
| 3 | 1.000 | 20.88 | 9.51 | 1.37e-3 | -2.86 | 2.8 | 1.44e-1 | -0.84 | |
| 14day-seedling | 1 | 0.999 | 25.2 | 11.63 | 3.16e-4 | -3.5 | 4.98 | 3.16e-2 | -1.5 |
| 2 | 0.999 | 26.43 | 8.32 | 3.12e-3 | -2.51 | 5.42 | 2.34e-2 | -1.63 | |
| 3 | 0.999 | 24.53 | 6.74 | 9.38e-3 | -2.03 | 4.6 | 4.13e-2 | -1.38 | |
| 25day-aerial | 1 | 1.000 | 23.61 | 10.76 | 5.78e-4 | -3.24 | 5 | 3.13e-2 | -1.5 |
| 2 | 1.000 | 25.19 | 11.15 | 4.40e-4 | -3.36 | 4.91 | 3.33e-2 | -1.48 | |
| 3 | 0.999 | 24.66 | 10.78 | 5.69e-4 | -3.24 | 5.22 | 2.69e-2 | -1.57 | |
| 35day-aerial | 1 | 0.999 | 24.61 | 10.16 | 8.76e-4 | -3.06 | 4.62 | 4.07e-2 | -1.39 |
| 2 | 1.000 | 23.81 | 11.09 | 4.59e-4 | -3.34 | 4.96 | 3.22e-2 | -1.49 | |
| 3 | 0.996 | 24.69 | 11.5 | 3.46e-4 | -3.46 | 5.31 | 2.52e-2 | -1.6 | |
| 42day-rosette | 1 | 1.000 | 24.68 | 11.14 | 4.44e-4 | -3.35 | 4.03 | 6.11e-2 | -1.21 |
| 2 | 0.999 | 27.76 | 13.38 | 9.40e-5 | -4.03 | 7.69 | 4.86e-3 | -2.31 | |
| 3 | 1.000 | 24.42 | 10.9 | 5.25e-4 | -3.28 | 5.33 | 2.49e-2 | -1.6 | |
| 42day-stem | 1 | 0.999 | 22.26 | 1.56 | 6.66e-1 | -3.18 | 5.25 | 2.62e-1 | -1.58 |
| 2 | 0.998 | 24.02 | 11.47 | 3.52e-1 | -3.45 | 5.92 | 1.65e-1 | -1.78 | |
| 3 | 1.000 | 24.55 | 11.17 | 4.36e-1 | -3.36 | 4.86 | 3.44e-1 | -1.46 | |
| 53day-rosette | 1 | 1.000 | 24.14 | 10.09 | 9.17e-4 | -3.04 | 4.1 | 5.82e-2 | -1.23 |
| 2 | 1.000 | 25.7 | 13.41 | 9.18e-5 | -4.04 | 7.7 | 4.83e-3 | -2.32 | |
| 3 | 1.000 | 27.6 | 14.71 | 3.73e-5 | -4.43 | 8.05 | 3.77e-3 | -2.42 | |
| 53day-stem | 1 | 0.999 | 24.74 | 11.31 | 3.93e-4 | -3.41 | 4.79 | 3.60e-2 | -1.44 |
| 2 | 0.999 | 27.86 | 13.09 | 1.15e-4 | -3.94 | 8.43 | 2.89e-3 | -2.54 | |
| 3 | 0.997 | 23.14 | 5.53 | 2.16e-2 | -1.67 | 2.58 | 1.67e-1 | -0.78 | |
| 53day-inflores | 1 | 0.999 | 23.24 | 9.85 | 1.08e-3 | -2.97 | 4.7 | 3.86e-2 | -1.41 |
| 2 | 1.000 | 25.4 | 10.77 | 5.73e-4 | -3.24 | 6.21 | 1.35e-2 | -1.87 | |
| 3 | 0.999 | 23.61 | 9.78 | 1.14e-3 | -2.94 | 5.19 | 2.74e-2 | -1.56 | |
| 53day-silique | 1 | 1.000 | 29.07 | 9.22 | 1.68e-3 | -2.78 | 5.27 | 2.59e-2 | -1.59 |
| 2 | 1.000 | 30.6 | 11.31 | 3.94e-4 | -3.4 | 7.6 | 5.14e-3 | -2.29 | |
| 3 | 1.000 | 30.41 | 11.44 | 3.61e-4 | -3.44 | 7.7 | 4.79e-3 | -2.32 | |
NGS expression data
Expression of miR160b transcript in NGS dataSequences
miRNA transcript
GUCGUGCCUGGCUCCCUGUAUGCCACAAGAAAACAUCGAUUUAGUUUCAAAAUCGAUCACUAGUGGCGUACAGAGUAGUCAAGCAUGACCAAAGCUUAUCAUAAUAUCUA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCUGGCUCCCUGUAUGCCA | miRNA | 5 | 25 | |
TGCCTGGCTCCCTGTATG | primer | 5p | 5 | 22 |
GATATTATGATAAGCTTTGGTCATGC | primer | 3p | 83 | 108 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR160b found in PubMed |